ABSTRACT: Introduction: The Herrmann Brain Dominance Instrument (HBDI) is an instrument designed to assess cognitive preferences based on the Whole Brain Model (logical-analytical, sequential-organized, interpersonal-emotional, and holistic-creative quadrants). Despite its international application, a validated adaptation for the Latin American context is absent. Aim: This study aimed to evaluate the psychometric properties of the Spanish adaptation of the HBDI (Jiménez, 2006) within a Latin American university student population. Methods: Data from 578 business university students were subjected to EFA to examine structural validity, internal consistency analysis for reliability and a MIMIC model to assess measurement invariance. Results: EFA yielded a reduced 11-item structure in Study 1, which was further refined to an 8-item solution (two items per quadrant) in Study 2. Discussion/Conclusion: The findings indicated robust factorial validity and acceptable internal consistency for each subscale provide evidence of the adapted HBDI for use within Latin American university settings.